ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Nucleotide and Derived Amino Acid Sequences of the Cyanogenic Beta-Glucosidase (Linamarase) from Cassava (Manihot esculenta Crantz))

ผู้เขียน:Prachumporn Toonkool, Nusra Tongtubtim

สื่อสิ่งพิมพ์:pdf

Abstract

Many isozymes of cassava cyanogenic ?-glucosidase (linamarase) exist, but only one cDNA sequence (pCAS5) has been reported thus far. In order to study the structure-function relationships in this enzyme, the cDNA of cassava linamarase was cloned and sequenced. In this report, six different cDNA sequences of linamarase from cassava were cloned by reverse transcription-polymerase chain reaction (RT-PCR) using primers designed from the sequence of pCAS5. Nucleotide sequences of all six clones showed 98-99% sequence identity to the nucleotide sequence of pCAS5. Derived amino acid sequences from four cDNA clones showed 98-99% sequence identity to that of pCAS5, while the other two cDNA clones contained nucleotide sequences that led to premature termination.

Article Info

Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 040, Issue 5, Jan 06 - Jun 06, Page 123 - 128 |  PDF |  Page